Allen Institute · 3 days ago
Scientist III – Immunologist, iPSC-derived allogeneic T-cell engineering
Seattle, WA
Full-time
Onsite
Mid, Senior Level
$128K/yr - $160K/yr
5+ years expThe Allen Institute is dedicated to unlocking the complexities of bioscience to improve human health. They are seeking an innovative immunologist to join their founding team at SeaBridge, a new translational incubator, to drive the allogeneic CAR-T cell therapy program and develop protocols for iPSC-derived T-cell lines.
Neuroscience
Responsibilities
Play a founding role in creating an incubator designed to turn discoveries into cures
Shape and lead a cutting-edge allogeneic (off-the-shelf) CAR-T cell therapy program
Drive innovation at the intersection of iPSCs, CRISPR, and translational science
Develop robust protocols for deriving T-cell lines from iPSCs and for applying state-of-the-art CRISPR/Cas9 gene editing technologies to enhance their therapeutic properties
Design, execute, and analyze gene editing experiments using CRISPR/Cas9 and other technologies to modify iPSC-derived T-cells
Maintain and expand high-quality human iPSC lines in a cGMP-compliant manner
Engineer T-cells to express chimeric antigen receptors (CARs) for targeted cancer therapy
Apply genome editing to remove or insert genes to enhance T-cell potency, reduce T-cell exhaustion, or mitigate off-target toxicity (e.g., knocking out TCR to prevent Graft-vs-Host Disease)
Use multi-color flow cytometry, cell sorting, and functional assays to characterize iPSC-derived and engineered T-cells
Assess T-cell functionality, such as proliferation, cytokine production, and antigen-specific cytotoxicity
Conduct molecular characterization using techniques like ddPCR, qPCR, Sanger sequencing, and next-generation sequencing to confirm successful gene editing
Work effectively in a cross-functional team, interacting with process development, analytical development, and regulatory affairs
Document all experimental procedures and results in electronic lab notebooks and technical reports
Present data and findings at internal meetings to advance CAR-T pipeline programs
Qualification
Required
Ph.D. in Immunology, Molecular Biology, Cell Biology, Bioengineering, or a related field; or equivalent combination of education and experience
5 years of relevant postdoctoral or industry experience or equivalent experience
Preferred
5 – 8 years relevant post-doctoral experience
Strong hands-on experience with human iPSC culture, maintenance, and differentiation protocols
Extensive practical experience with CRISPR/Cas9 or other gene editing technologies in mammalian cells
Proficiency in primary human T-cell culture and engineering is a strong plus
Expertise in multi-color flow cytometry and cell sorting for cell characterization
Experience with viral (lentivirus, AAV) and non-viral gene delivery methods
Excellent communication and interpersonal skills
Ability to work independently and as part of a collaborative, fast-paced team
Strong problem-solving and troubleshooting abilities
Demonstrated team management
Demonstrated ability to build and lead a program
Benefits
Medical, dental, vision, and basic life insurance
401k plan
Paid time off
Company
Allen Institute
The Allen Institute is dedicated to answering some of the biggest questions in bioscience and accelerating research worldwide.
501-1000 employees
H1B Sponsorship
Allen Institute has a track record of offering H1B sponsorships. Please note that this does not
guarantee sponsorship for this specific role. Below presents additional info for your
reference. (Data Powered by US Department of Labor)
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Trends of Total Sponsorships
2025 (41)
2024 (33)
2023 (25)
2022 (26)
2021 (17)
2020 (7)
Funding
Current Stage
Late StageLeadership Team
Rui Costa
President and CEO
Andy Hickl
Chief Technology Officer
Recent News
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