Apply on Employer Site

Alaka`ina Foundation Family of Companies · 1 week ago

Senior Bacterial Core Technician

Frederick, MD

Contract

Onsite

Senior Level

5+ years exp

The Alaka`ina Foundation Family of Companies is a fast-growing government service provider seeking a Senior Bacterial Core Technician to support their government customer at Fort Detrick, Frederick, MD. The role involves managing BSL-3 laboratory operations, conducting various animal studies, and performing in vitro testing of therapeutic compounds against Tier 1 bacteria.

CharityCommunitiesGovernment

No H1B

U.S. Citizen Only

Responsibilities

Responsible for BSL-3 laboratory operations and training in animal manipulations. Technicians shall perform approximately 5 animal studies in both the DTRA funded projects as well as contracted work

Perform in vitro Minimum Inhibition Concentration (MIC) testing of potential therapeutic compounds against Tier 1 bacteria. We currently utilize 30-strain diversity sets of Bacillus anthracis (anthrax), Yersinia pestis (plague), Francisella tularensis (tularemia), Burkholderia mallei (glanders), and Burkholderia pseudomallei (meliodosis), testing over 72 compounds per year against all 30 strains, in vitro

Participate in mouse, rat, rabbit, or other small animal studies, which include preparation of antibiotics for administration, administering drugs and vaccines, orally and by intraperitoneal, intramuscular, subcutaneous, and intravenous bolus injections. In addition, collect animal body fluids and remove tissues with all small animal studies executed. Upon execution of experiment shall perform animal health exams to ensure study remains in compliance with IACUC approved protocols

Participate in weekly lab meetings and assist in development and design with current and future in vitro and in vivo work. May be requested to present posters at 1 scientific conference which may require CONUS or OCONUS travel and work to be performed outside normal business hours

Participate in mouse, rat, rabbit, or other small animal BSL-2 pharmacokinetics and toxicology studies; to include preparation of antibiotics for administration; administering drugs and vaccines, followed by blood and tissue collection for pharmacokinetic or pharmacodynamic analyses

Support the in vivo pharmacology research program and contribute to the multidisciplinary research team to determine pharmacokinetics and pharmacodynamics relationships and efficacy of optimized leads

Assist with data analyses and drafting reports for internal or external customers

Work with mass spectrometry, chromatography, HPLC MS/MS, specifically Sciex platforms coupled with Shimadzu HPLCs other and biophysical techniques to support in-process testing and characterization of biopharmaceutical products and impurities

Perform bioanalytical data acquisition, data analysis, and reporting of results using Applied Biosystems Analyst, Excel, and the Mass Spec Quantitation Software MultiQuant

Complete approximately 25 media/reagent preparations per year

Assist with approximately 5 animal challenges per year and 10 bacterial challenge preparations

Perform approximately 400 animal injections per year; 400 animal observations per year

Support divisional in vivo projects by completing approximately 500 bacterial burdens in animal tissue per year

Perform approximately 500 small animal necropsies per year

Process approximately 300 animal gavage and approximately 125 animal blood collections per year

Place approximately 50 GFEBs orders per year for supplies and equipment

Maintain laboratories in BSL-2 and BSL-3

Perform or assist with BSAT Inventory Management weekly (to include applicable BSAT working stock material)

Conduct or assist with Equipment Inventory Management twice a year in BSL-2 and BSL-3

Perform or assist with monthly Laboratory Management to include safety functions and chemical inventory

Review relevant literature monthly. May assist in the preparation standard operating procedures and manuscripts relating to their research efforts. May be expected to present research findings at one scientific conference per year, which may require CONUS or OCONUS travel. Present results in written and oral format publicly and internally as requested

Maintain a GLP training folder and be compliant with all GLP regulations when applicable

Directly contribute to the design, rationale, and be point-of-contact for 10 small animal studies to include drafting of a study plan and oversight of personnel scheduling

Contribute to study progress reports and scientific manuscripts to include statistical analysis, data compilation, figure preparation, and methods summary

Assist in establishing up to 28 new active material transfer agreements and cooperative research and development agreements with up to 8 pharmaceutical companies and/or universities. Maintain records of active agreements and collaborations

Other duties as assigned by Supervisor

Qualification

BSL-3 laboratory operationsIn-vitro MIC testingAnimal studiesPharmacology researchMass spectrometryHPLC MS/MSApplied Biosystems AnalystLaboratory ManagementSoft skills

Required

Bachelor's degree in Biology, Microbiology or similar science and 5 years of relevant laboratory experience

Must have a minimum of two (2) to three (3) years performing BSL-3 laboratory operations and training in animal manipulations and conducting animal studies

Must have a minimum of two (2) to three (3) years performing in-vitro Minimum Inhibition Concentration (MIC) testing of potential therapeutic compounds against Tier 1 bacteria

Must be willing to work with diversity sets of Bacillus anthracis (anthrax), Yersinia pestis (plague), Francisella tularensis (tularemia), Burkholderia mallei (glanders), and Burkholderia pseudomallei (meliodosis)

Must have a minimum of three (3) to five (5) years conducting small animal studies (mouse, rat, rabbit, or other) which include preparation of antibiotics for administration, administering drugs and vaccines, orally and by intraperitoneal, intramuscular, subcutaneous, and intravenous bolus injections, collecting animal body fluids and removing of tissue samples

Must have a minimum of two (2) to three (3) years' experience working within IACUC approved protocols

Must have research experience in pharmacology, pharmacokinetics, and pharmacodynamics

Must have direct experience in in-process testing and characterization of biopharmaceutical products and impurities using mass spectrometry, chromatography, HPLC MS/MS

Must have a minimum of two (2) to three (3) years' experience with Applied Biosystems Analyst, Excel, and the Mass Spec Quantitation Software MultiQuant

Must have a minimum of two (2) to three (3) years performing Animal Necropsies, animal gavage and animal blood collections

Must have a minimum of two (2) to three (3) years performing Equipment Inventory Management

Must have a minimum of two (2) to three (3) years performing Laboratory Management within BSL-2 and BSL-3 environments, to include safety functions and chemical inventory

U.S. Citizen

BPRP and CS-PRP enrollment required

Must pass NACI background check