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Goldbelt Apex LLC · 2 hours ago

Senior Bacteriology Technician

Fort Detrick, MD

Full-time

Onsite

Senior Level

$80K/yr - $90K/yr

7+ years exp

Goldbelt Apex LLC is a data-focused company dedicated to process and quality in healthcare IT. The Senior Bacteriology Technician will assist in molecular microbiological experiments and support various laboratory tasks related to bacterial virulence and immunological analyses.

Responsibilities

Shall prepare approximately 10 different Reagents / Media for the lab (including R Medium, DMEM/FBS, LB Broth, Sodium Bicarbonate, APS LB Agar, BHI, etc)

Contractor shall plate approximately 100 LB plates for use for Ames sporulation isolation and purification

Contractor shall isolate and purify approximately 10 plasmid DNA specimens

FITC label and count approximately 10 Ames samples to check ratio of Bacilli: Spores over multiple time points

Contractor shall order lab supplies for BSL-2 and BSL-3 laboratories including common supplies, custom orders, and equipment - approximately 400 items ordered along with 5 pieces of equipment

Present overview of data collected from Opsonic Adherence and ELISA at group meeting; present progress reports to supervising investigator. Use computer to prepare graphs and write reports for supervising investigator

Contractor shall perform approximately 100 Immunological, antibody determinations

Contractor shall perform approximately 100 ELISAs on mouse, nonhuman primate, and/or human serum

Contractor shall perform approximately 50 Cytokine analyses. Measures cytokines in mouse, nonhuman primate, and/or human serum. Measures cytokines in cell cultures and cell extracts. Uses a Luminex instrument for analysis

Contractor shall perform approximately 50 Flow Cytometry tasks, determining cellular distribution in mouse samples and intracellular cytokine expression. Uses a BD FACS Calibur and BD FACS Canto II to analyze cellular distribution

Perform other duties as necessary