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Glyphic Biotechnologies · 2 months ago

Senior/Staff Scientist, Assay Development (Sample Prep)

Berkeley, CA

Full-time

Onsite

Senior Level, Lead/Staff

$142K/yr - $183K/yr

6+ years exp

Glyphic Biotechnologies is developing a transformative protein sequencing platform and is seeking a Senior/Staff Scientist to lead sample preparation and support assay development. The role involves designing and optimizing experimental protocols, ensuring high throughput and integration with chemistry developments to enable robust sequencing solutions.

BiotechnologyHealth CareLife Science

Responsibilities

Design, validate, and optimize ProSE (proprietary single molecule protein sequencing platform) experiments for robustness, sensitivity, and scalability, including improving reaction efficiency and kinetics in mixed phase assays

Lead the integration of chemistry developments into assay applications, identifying and addressing potential incompatibilities

Optimize and automate c-terminal and side chain blocking chemistries provided by other teams for use in the assay

Propose and test high-sensitivity molecular tools to build predictive understanding of factors affecting assay robustness and sensitivity

Evaluate and process incoming KOL (Key Opinion Leader) samples, ensuring sample preparation methods accommodate specific constraints and requirements

Assess diverse sample types, determine appropriate analytical approaches, and develop protocols for extraction and purification (cells, tissue), collaborating with chemists to scale and integrate into high-throughput workflows

Own the introduction and validation of real biological sample inputs to enable sequencing of biologically relevant material, including complex and challenging samples

Develop and implement strategies for sample enrichment, depletion, and dynamic range management to optimize assay performance

Collaborate with early access KOLs to ensure seamless integration of sample workflows and address unique sample challenges

Qualification

Assay developmentSingle molecule assaysSample preparation methodsHigh sensitivity multiplexed quantificationBiological sample experienceProblem-solving skillsCommunication skillsInterpersonal skillsResourceful adaptabilityCollaborative spirit

Required

Degree in biochemistry, chemical biology, or equivalent field. Advanced degree preferred

6+ years (PhD), 8+ years (MS), 10+ years (B.S.) of industry or equivalent experience in assay development

Expertise in developing and optimizing single molecule or ultrasensitive molecular assays

Deep understanding of high sensitivity highly multiplexed protein quantification (nELISA, NuLISA, immunoassays, PLE, PEA)

Expertise in developing sample prep or enrichment methods for low abundance targets or input material

Experience working with real biological samples (non-contrived) and developing assays that account for matrix variability and real-world conditions

Excellent problem-solving skills and attention to detail

Excellent written and verbal communication skills, with the ability to work seamlessly with colleagues from diverse scientific and technical backgrounds

Ability to thrive in a fast-paced environment with evolving priorities