Alnylam Pharmaceuticals · 2 months ago
Principal Associate Scientist, RNAi Discovery
Cambridge, MA
Full-time
Hybrid
Senior Level, Lead/Staff
$94K/yr - $141K/yr
5+ years expAlnylam Pharmaceuticals is seeking a Principal Associate Scientist to join their High Throughput In Vitro team within the RNAi Discovery group. The role involves conducting in vitro research studies, managing high-throughput experiments, and contributing to the development of RNAi therapeutics.
BiotechnologyHealth CareMedicalPharmaceuticalTherapeutics
Responsibilities
Conduct a wide range of in vitro siRNA research studies to evaluate RNAi compounds using biochemical, molecular, and cell-based assays
Plan, coordinate, and execute day-to-day high-throughput in vitro experiments, including transfection, RNA extraction, qPCR, data upload, and statistical analysis
Design, perform, and interpret experiments independently and collaboratively, providing critical troubleshooting and technical insight
Maintain and culture various cell types (iPSCs, primary cells, immortalized cell lines) to support research objectives and facilitate high-throughput siRNA screening through qPCR and cell-based assays
Ensure accurate data recording, analysis, and timely reporting, preparing clear and detailed experimental summaries, documentation, and presentations
Identify and resolve technical challenges, contributing to the ongoing optimization and development of experimental protocols
Operate and maintain automated instrumentation and robotic systems to support high-throughput (384-well) screening workflows
Qualification
Required
BS/MS in Biology, Molecular/Cell Biology, Biochemistry, or related field with 5–8 years of relevant experience, preferably in an industrial or biotech environment
Proficiency in mammalian cell culture, including maintenance and transfection of immortalized and primary cell lines
Skilled in molecular biology techniques for RNA, DNA, and protein quantification (qPCR, dPCR, Western blotting, ELISA, immunoprecipitation)
Strong knowledge of experimental design, data interpretation, and scientific troubleshooting
Highly detail-oriented, organized, and capable of managing multiple projects in a fast-paced, dynamic environment
Demonstrated ability to collaborate and communicate effectively with cross-functional teams and stakeholders
Proficiency with Microsoft Office (Excel, Outlook, PowerPoint, Word) and GraphPad Prism
Preferred
Prior experience or strong understanding of nucleic acid–based therapeutics (siRNA, ASO, CRISPR) highly desired
Hands-on experience with laboratory automation systems, including liquid-handling platforms such as PerkinElmer JANUS, Beckman i7, or similar
Benefits
Comprehensive benefits including medical, dental, and vision coverage
Life and disability insurance
A lifestyle reimbursement program
Flexible spending and health savings accounts
A 401(k) with a generous company match
Paid time off
Wellness days
Holidays
Two company-wide recharge breaks
Generous family resources and leave
Company
Alnylam Pharmaceuticals
Alnylam Pharmaceuticals is a biopharmaceutical company focused on the discovery, development and commercialization.
1001-5000 employees
H1B Sponsorship
Alnylam Pharmaceuticals has a track record of offering H1B sponsorships. Please note that this does not
guarantee sponsorship for this specific role. Below presents additional info for your
reference. (Data Powered by US Department of Labor)
Distribution of Different Job Fields Receiving Sponsorship
Represents job field similar to this job
Trends of Total Sponsorships
2022 (18)
2021 (19)
2020 (7)
Funding
Current Stage
Public CompanyTotal Funding
$4.55BKey Investors
Blackstone Life SciencesBlackstone CreditRegeneron
2025-09-09Post Ipo Debt· $661.25M
2022-09-12Post Ipo Debt· $900M
2022-03-04IPO
Leadership Team
Yvonne Greenstreet
Chief Executive Officer
Jeff Poulton
Chief Financial Officer
Recent News
Longevity.Technology
2026-01-14
2026-01-13
Company data provided by crunchbase