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Fred Hutch · 4 hours ago

Staff Scientist

Seattle, WA

Full-time

Onsite

Senior Level, Lead/Staff

$84K/yr - $192K/yr

Fred Hutchinson Cancer Center is an independent, nonprofit organization focused on cancer treatment and research. The Staff Scientist will join the translational proteomics team to drive projects forward by performing experiments and analyses of clinical and preclinical biospecimens.

Hospital & Health Care

No H1B

Responsibilities

Process proteomic samples for mass spectrometry analysis (e.g., protein extraction, fractionation, immuno-depletion, trypsin digestion, alkylation, reduction, etc.)

Assist in generation and analysis of mass spectrometry-based proteomic data

Follow standard operating protocols (SOP) in a CLIA/CAP laboratory environment including participating in annual competency and proficiency testing

Work with antibodies (antibody crosslinking/purification, immunoaffinity purification, immobilization on solid supports, ELISAs)

Collaborate effectively with an interdisciplinary team spanning expertise in medicine, biology, analytical chemistry, and statistics

Design and present Powerpoint presentations of results to colleagues and collaborators

Maintain laboratory equipment, records, research results files, and databases

Write progress reports and develop standard operating protocols (SOPs)

Write and co-author scientific manuscripts (in peer-reviewed journals)

Perform other duties as required

Qualification

Mass SpectrometryProteomicsAnalytical ChemistryHPLCCLIA/CAP EnvironmentData Analysis ToolsPythonWillingness to LearnCommunicationTeam CollaborationAttention to Detail

Required

Ph.D. degree in Protein Chemistry, Analytical Chemistry, Biochemistry, or related discipline

Previous work maintaining and running mass spectrometry and HPLC instruments to generate proteomic data

Strong work ethic and ability to multi-task efficiently in a highly interactive, interdisciplinary team environment

Comfort in a milestone-driven environment, with quarterly objectives

Exquisite attention to detail and the ability to adhere to standard operating protocols (SOP) and maintain records in a CLIA/CAP environment

Proficiency with MS Office programs (Word, Powerpoint, Excel, and Access)

Willingness to learn new techniques

Excellent English writing and oral communication skills

Preferred

Previous experience in a CLIA/CAP or other regulated environment

Previous experience with Tandem Mass Tag (TMT) labeling, data-dependent acquisition (DDA) and data-independent acquisition (DIA) mass spectrometry techniques

Previous experience with multiple reaction monitoring (MRM) and parallel reaction monitoring (PRM) targeted mass spectrometry techniques

Previous experience isolating human peripheral blood mononuclear cells (PBMC) for proteomics experiments- isolation from whole blood, expansion in culture, perturbation with chemical agents, protein extraction and quantification, etc

Previous experience with QC and processing of formalin-fixed paraffin-embedded (FFPE) and flash-frozen specimen processing and protein extraction for proteomics experiments

Previous experience in mass spectrometry data analysis tools (such as Proteome Discoverer, Fragpipe, Skyline, etc.)

Previous experience with quantitative image analysis for pathology (such as Indicta Labs HALO software)

Previous work with antibodies and peptide standards (immunoaffinity purification, immobilization on solid supports, preparation of samples for mass spectrometry)

Previous experience with liquid handling robot usage and programming (Python, R)

Ability to perform traditional biochemistry experiments on human and animal biospecimens and cell lines

A strong publication record