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Scottish Rite for Children · 2 hours ago

Research Associate

Dallas, TX

Full-time

Onsite

Senior Level

5+ years exp

Scottish Rite for Children is committed to giving children back their childhood, and they are seeking a Research Associate. This role involves various laboratory responsibilities including cell processing, molecular biology techniques, imaging, and data analysis to support pediatric research initiatives.

ChildrenHealth CareHospitalMedical

Comp. & Benefits

Responsibilities

Perform processing of blood and bone marrow samples, including filtration, Ficoll gradient separation, and magnetic-activated cell sorting (MACS)

Isolate and culture bone marrow stromal cells (BMSCs); perform osteogenic induction and staining procedures such as Alizarin Red and alkaline phosphatase (ALP) staining

Maintain macrophage cultures, including routine medium changes, stimulation, detachment/harvesting, and cryopreservation

Apply molecular biology techniques such as single-cell cloning and cell line development

Perform genome-editing experiments using CRISPR/Cas9 systems, RNA interference (RNAi), or other gene-modulation tools

Validate edited or cloned cells through PCR genotyping, sequencing, and phenotype-based assays

Contribute to optimization of cloning, transfection, and gene-editing workflows

Conduct micro-CT scanning, image reconstruction, and quantitative data analysis

Utilize microscopes for live/dead viability staining, routine culture monitoring, and morphological assessments

Perform RNA extraction, cDNA synthesis, and quantitative PCR (qPCR) for gene-expression analysis

Conduct biochemical assays including ELISA, BCA protein quantification, Western blot analysis, and MTT viability assays

Prepare samples, reagents, and staining panels for flow cytometry (FACS) analysis

Analyze imaging and experimental data using CTAn, CTVolX, FlowJo, ImageJ/Fiji, Excel-based qPCR analysis tools, and GraphPad Prism

Maintain accurate electronic records and generate figures, data summaries, and reports

Prepare, track, and manage chemical, reagent, and supply orders; complete order forms and submit purchasing requests

Assist with maintaining laboratory organization, equipment maintenance, and routine quality-control documentation

Qualification

Cell ProcessingMolecular BiologyGene EditingImaging TechniquesBiochemical AssaysData Analysis SoftwareMulti-taskingLaboratory OperationsImmunology ExperienceCritical ThinkingTime Management

Required

Bachelor's degree with 10 years of laboratory experience, or master's degree with 5 years of laboratory experience required

Strong critical thinking and time-management skills

Ability to multi-task and work with minimal supervision in a research environment

Preferred

Experience with cell cloning and gene editing is preferred

Prior experience in immunology or bone biology is strongly preferred. (inflammation, macrophage biology, or immuno-modulation)

Company

Scottish Rite for Children

Scottish Rite for Children is a world-renowned leader in the treatment of pediatric orthopedic conditions with the focus of giving children back their childhood through expert care.

Founded in 1921

Dallas, Texas, USA

1001-5000 employees