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Kelly Science, Engineering, Technology & Telecom · 3 weeks ago

Bioengineer - NIH - NIAID

Gaithersburg, MD

Full-time

Onsite

Mid, Senior Level

2+ years exp

Kelly Government Solutions is a strategic partner to the federal government, seeking qualified individuals to work as a Bioengineer for the National Institutes of Health (NIH). The role involves developing microfluidics-based approaches for analyzing intra-host virus diversity and evolution, collaborating with scientists, and maintaining records of work using appropriate electronic systems.

Staffing & Recruiting

Responsibilities

Develop microfluidics-based approaches for analysis of intra-host virus diversity and evolution, such as:

An approach for high-throughput, single-copy, full-length sequencing of purified virus genomes, to include the following:

Optimized virus inactivation and nucleic acid extraction procedures from blood and body fluids

Customized microfluidic devices, regimes of operation, and reagents that partition extracted virus genomes in aqueous solution into monodisperse water-in-oil emulsions and thus enable downstream single-copy analysis

An approach for high-throughput sequencing of individual intact virions, to include the following:

Non-destructive procedures for abrogating the infectivity of intact virions in suspension

Customized microfluidic devices, regimes of operation, and reagents that can partition single-virion suspensions into water-in-oil emulsions at limiting dilution and thus enable downstream single-virion analysis

Maintain records of all work using appropriate electronic systems

Present results at internal and external meetings

Collaborate with NIH and non-NIH scientists

Qualification

Microfluidic device designPacific Biosciences RevioRNA extractionLaboratory automationBioinformatics toolsHuman immunologyPeer-reviewed publicationsCollaboration skills

Required

Ph.D. in bioengineering, mechanical engineering, or physics and 2+ years of experience

Professional competency with design and fabrication of microfluidic devices, including flow-based droplet generators, using soft lithography (i.e., template design/production and device generation in polydimethylsiloxane [PDMS]) and glass fabrication techniques

Professional competency with operation of the Pacific Biosciences Revio single-molecule real-time (SMRT) sequencer platform

Professional competency with basic bioinformatic and evolutionary biology tools for sequence analysis, including current software for database searches, multiple sequence alignment generation, and phylogenetic inference

Professional competency with RNA extraction from human blood and body fluid samples, reverse transcription, polymerase chain reaction, and sequencing library preparation and quality control

Intensive experience with laboratory automation, including liquid handling systems

Experience with the application of complex bioinformatic pipelines for next-generation sequence data analysis

Working understanding of human immunology, with emphasis on innate and adaptive antiviral host responses

Track record of high-profile, peer-reviewed, first-author publication in the field of bioengineering

Track record of high-profile, peer-reviewed, first-author publication in the field of virus evolution

Track record of high-impact publications related to RNA virus infections

Benefits

Competitive compensation and comprehensive benefit package

Optional health, vision, and dental plans

Vacation leave as well as 10 paid federal holidays and 401K plan

Access to NIH’s unparalleled resources and niche scientific initiatives

Voluntary benefit plans including medical, dental, vision, telemedicine, term life, whole life, accident insurance, critical illness, a legal plan, and short-term disability

Retirement savings plan

Service bonus and holiday pay plans (earn up to eight paid holidays per benefit year)

Transit spending account

Paid sick leave under the applicable state or local plan