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CHEManager International · 3 hours ago

Laboratory Research Technician IV

Pittsburgh, PA

Full-time

Onsite

Mid, Senior Level

CHEManager International is focused on investigating mechanisms linking inflammation, autophagy, and carcinogenesis in colorectal cancer patients. They are seeking a Laboratory Research Technician IV to support their translational research team by performing various molecular and cellular experiments and managing complex workflows independently.

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Responsibilities

Perform and oversee molecular and cellular experiments, including DNA/RNA extraction, PCR, Western blotting, and ELISA

Conduct murine studies including genotyping, sample collection (colon, lymphoid organs), tissue processing, and histology preparation (FFPE, frozen)

Establish and maintain primary epithelial and immune cell cultures, including murine and human colonic organoids, and perform co-culture and drug treatment assays

Execute and assist with immunofluorescence and immunohistochemistry

Assist in flow cytometry and single-cell RNA sequencing sample preparation

Maintain detailed electronic lab notebooks and organize experimental data for analysis and publication

Support the PI and trainees in data analysis, figure preparation, and manuscript writing

Manage laboratory inventory, ordering, and compliance with safety protocols; serve as lab safety liaison as needed

Train and mentor junior staff, students, or fellows in lab techniques

Qualification

Molecular biologyHistologyCell-based assaysDNA/RNA extractionPCRWestern blottingELISAFlow cytometrySingle-cell RNA sequencingData analysisFigure preparationManuscript writingLab safety complianceTrainingMentoring

Required

Experienced in molecular biology, histology, and cell-based assays

Capable of managing complex workflows independently

Comfortable in both mouse and human tissue research settings