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Nesco Resource · 1 hour ago

Scientist - II

Rahway, NJ

Full-time

Onsite

Mid Level

$50/hr - $53.45/hr

3+ years exp

Nesco Resource is seeking a hands-on scientist to execute high-throughput cell-based assays in 384-well format. The role involves culturing and plating cells, dispensing compounds, running detection readouts, and analyzing dose-response data to deliver high-quality EC50 results for discovery programs.

ConsultingHuman ResourcesStaffing Agency

Responsibilities

Maintain, expand, and plate mammalian cell lines for 384-well assays

Set up and execute assays with compound dispensing via Labcyte/Beckman Echo

Run and optimize detection formats (e.g., luminescence, cAMP, HTRF/TR-FRET, fluorescence)

Generate dose–response curves (4-parameter logistic fits) and report EC50/IC50 with QC

Troubleshoot assay performance, optimize Z' factors, and document SOPs/results

Collaborate with discovery teams to prioritize studies and timelines

Additional responsibilities may include setting up western blot assays and learning how to run in-cell westerns

Qualification

Mammalian cell culture384-well assay workflowsData analysis skillsPlate readers experienceAutomation/LIQ handlersGraphPad Prism familiarityOrganizedDetail-orientedFast-paced environment

Required

BS/MS/PHD in Biology, Pharmacology, Biochemistry, or related field; BS with 5+ years, Masters with 3+ years and PHD with 1+ year of industry or equivalent lab experience

Proficiency in mammalian cell culture and 384-well assay workflows

Experience with plate readers and assay platforms (e.g., cAMP, HTRF, luminescent assays)

Strong data analysis skills: 4PL curve fitting, EC50/IC50 determination, and curve QC (e.g., R^2, top/bottom constraints)

Detail-oriented, organized, and comfortable in a fast-paced, high-throughput environment