Eurofins PSS Insourcing Solutions · 1 day ago
Associate Scientist, Gene Editing and iPSC Engineering
Boston, MA
Full-time
Onsite
Mid Level
$37/hr - $41/hr
3+ years expEurofins PSS Insourcing Solutions is a global leader in life sciences, providing analytical testing services across various industries. They are seeking a highly skilled Associate Scientist to join their diabetes team, focusing on advancing iPSC stem cell engineering and developing islet cell therapies through gene editing and molecular biology techniques.
Biotechnology
No H1B
Responsibilities
Enabling and advancing iPSC gene editing, cell line development, and evaluation of differentiated cells
Routine iPSC cell culture, passaging, and media making
Optimize workflows for iPSC CRISPR based gene-editing, gRNA screening, and delivery
Optimize and perform assays for on-/off-target analysis via PCR and NGS workflows
Generate clonal gene edited iPSC lines and characterize via flow cytometry, ddPCR, dPCR and qPCR
Culture and execute routine directed differentiation of human iPSCs to pancreatic islets performing drug substance assessments such as cell counts, viability, and metabolite assessments
Assist scientists in the team to evaluate and design mitigation strategies to ensure genomic stability of the iPSC lines and drug substance
Collaborate cross-functionally to support transfer of R&D assays to GMP
Maintain accurate and detailed records of all experiments, synthesize results, prepare reports, and present in meetings. Contribute to SOP development
Work seamlessly in executing workplans and meeting deadlines
Qualification
Required
B.S. in Stem Cell Biology, Cell Biology, Bioengineering or a related field with 3-4 years relevant industry experience in gene editing and cell therapy. M.S. in Stem Cell Biology, Cell Biology, Bioengineering or related field with 1-2 years relevant industry experience in gene editing and cell therapy
Extensive hands-on experience with human iPSC culture, gene editing, and molecular biology techniques
Proficiency in CRISPR gene editing and cell line development, including optimization of protocols, familiarity with flow-based sorting
Proficiency in molecular biology techniques including construct design, PCR, ddPCR, dPCR, and NGS
Hands-on experience with assay development including luciferase assays, ELISA, and flow cytometry
Hands-on experience with cell delivery methods of gene editing tools
Excellent communication and interpersonal skills, with the ability to work effectively in a team-oriented environment
Strong organizational skills and diligent record keeping
Preferred
Hands-on experience in pancreatic islet directed differentiation
Hands-on experience in transitioning research-grade processes to GMP-grade
Familiarity with electronic lab notebook (ELN) documentation
Authorization to work in the United States indefinitely without restriction or sponsorship
Benefits
Excellent full-time benefits including comprehensive medical coverage, dental, and vision options
Life and disability insurance
401(k) with company match
Paid vacation and holidays
Yearly goal-based bonus & eligibility for merit-based increases
Company
Eurofins PSS Insourcing Solutions
Welcome to Eurofins Professional Scientific Services® (PSS), where we redefine industry norms in insourcing solutions, creating unparalleled value for both our dedicated employees and esteemed clients.
1001-5000 employees
Funding
Current Stage
Late StageCompany data provided by crunchbase