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Glyphic Biotechnologies · 1 day ago

Proteomics Platform Scientist (Senior/Staff/Senior Staff/Principal)

Berkeley, CA

Full-time

Onsite

Senior Level

$146K/yr - $234K/yr

4+ years exp

Glyphic Biotechnologies is developing a revolutionary proteome sequencing platform aimed at transforming life science discovery. The Single-Cell Proteomics Platform Scientist will be responsible for building workflows that connect protein capture to sequencing, collaborating with diverse teams to ensure successful execution of experimental designs.

BiotechnologyHealth CareLife Science

Responsibilities

Develop bead-compatible chemistries to capture cell-surface and membrane-associated proteins, using both sequence-specific oligo strategies and non-oligo capture approaches, making first-principles design decisions around specificity, background, scalability, and downstream compatibility

Build robust protein capture workflows, including cleanup, validation, and yield/background optimization, owning reagent architecture and defining acceptance criteria

Integrate barcoding workflows with the downstream sequencing readout. Design the controls required to detect cross-talk, contamination, loss, and nonspecific binding

Benchmark against commercial surface kits and standard membrane enrichment methods. Identify failure modes and implement fixes

Design and execute proof-of-concept studies in bulk and in rare or defined populations

Document protocols and results in a form that can be transferred across organizations

Contribute to invention disclosures and external technical materials when warranted

Qualification

Bead-compatible capture chemistriesProtein chemistryOligo workSingle-cell workflow mindsetMammalian cell workBenchmarkingChemical functionalizationC-terminal conjugationYeast biologyTeam dynamicsSound judgmentResourceful adaptabilityProblem-solving

Required

Industry experience relevant to the role and an advanced degree in molecular biology, biochemistry or a related field, including: MS/PhD with 14+/10+ years (for Principal Scientist), MS/PhD with 12+/8+ years (for Senior Staff Scientist), MS/PhD with 8+/6+ years (for Staff Scientist), MS/PhD with 6+/4+ years (for Senior Scientist)

Bead-compatible capture chemistries: demonstrated expertise developing and optimizing bead-based capture of cell-surface and membrane-associated proteins using both sequence-specific oligo strategies (e.g., DNA-barcoded antibodies, oligo-handle docking systems) and non-oligo approaches (e.g., streptavidin capture of surface-biotinylated proteins, antibody-coated bead immunocapture)

Protein chemistry: hands-on conjugation and labeling (including antibody/affinity-reagent labeling and end-to-end protein–DNA/RNA conjugation) with practical validation methods, cleanup/QC, yield/background optimization, and strong troubleshooting instincts

Oligo work: comfortable designing and handling DNA oligos for capture or indexing, integrating barcoding workflows into an NGS readout (library prep and QC fundamentals), and building controls; proficient with bead-based DNA/protein purification and QC methods (e.g., gel extraction, TapeStation/Bioanalyzer, SeqStudio, UV-vis)

Mammalian cell work: cell culture, counting/viability, basic phenotyping, permeabilization and lysis, and clean experimental execution on cell lines and primary cells, including proof-of-concept studies from bulk to rare/low-input or enriched/sorted populations

Single-cell workflow mindset: demonstrated single-cell assay experience; you understand loss, background, nonspecific binding, and batch effects as first-order problems, and you design rigorous control strategies to quantify and mitigate cross-talk, contamination, loss, and background

Benchmarking: track record benchmarking against commercial surface profiling kits and standard membrane enrichment methods, diagnosing failure modes, and implementing fixes

Preferred

Experience with chemical functionalization of biomolecules or surfaces (e.g. EDC, NHS, Click)

C-terminal conjugation and modification of amino acid sidechains beyond cysteine and lysine

Basic experience with yeast biology, with the ability to culture yeast and support straightforward modification or process work needed for reagent prototyping or scale prep