Biohub · 1 day ago
Postdoctoral Fellow, Single Cell Method Development
New York, NY
Full-time
Onsite
Mid, Senior Level
$93K/yr - $93K/yrBiohub is leading the new era of AI-powered biology to cure or prevent disease through its 501c3 medical research organization, with the support of the Chan Zuckerberg Initiative. They are seeking a highly motivated Postdoctoral Fellow to lead the development of a high-throughput adaptation of a newly established single-cell nascent RNA sequencing method and apply it to patient biopsies and organoid models.
BioinformaticsBiotechnologyGenetics
Responsibilities
Assay development: Design, optimize, and scale high-throughput single-cell/nuclei extraction, barcoding, and library preparation workflows for scGRO-seq
Perturbation integration: Combine scGRO-seq with CRISPR-based enhancer perturbation, drug treatments, and multi-omics profiling
Molecular biology techniques: Execute advanced methods including assay design, cell manipulation, RNA/DNA isolation, PCR/qPCR, NGS library construction, high-dimensional flow cytometry, and sequencing
Translational applications: Adapt and validate protocols in patient biopsies and 3D organoid models, with emphasis on reproducibility and sensitivity
Immune cell engineering: Isolate, culture, and reprogram hematopoietic stem/progenitor cells (HSPCs) and primary immune subsets
Data integration: Partner with computational biologists to process and interpret large-scale single-cell datasets (RNA, chromatin, proteomics)
Collaboration & dissemination: Collaborate across disciplines to investigate regulatory genomics in immune dysfunction, tumor-infiltrating immune cells, and autoimmune disease variants. Contribute to publications, preprints, conference presentations, and grant proposals
Qualification
Required
PhD in Molecular Biology, Systems Biology, Genetics, Biomedical Engineering, or related field
Proven expertise and demonstrated success in bulk or single-cell method development
Experience designing and executing CRISPR screens and perturbation assays (genetic or chemical/drug)
Strong problem-solving skills, independence, and the ability to troubleshoot complex workflows
Excellent communication skills and proven ability to work in collaborative, multidisciplinary teams
Preferred
Experience and broad domain knowledge in microfluidic-based single-cell methods
Experience with hydrogel synthesis, single-cell barcoded bead generation, and aqueous droplets in oil emulsions
Experience with functional genomics in immune cells
Experience integrating multi-modal single-cell datasets (RNA, ATAC, proteomics)
Working knowledge of Linux/HPC environments and single-cell analysis tools (e.g., Seurat, Scanpy)
Benefits
Provides a generous employer match on employee 401(k) contributions to support planning for the future.
Paid time off to volunteer at an organization of your choice.
Funding for select family-forming benefits.
Relocation support for employees who need assistance moving
Company
Biohub
Our mission is to help scientists cure or prevent all disease.
Founded in 2015
201-500 employees
Funding
Current Stage
Growth StageLeadership Team
Rafael Gómez-Sjöberg
Chief Technology Officer
Eunitz Beganovic
Executive Assistant to the Chief Legal Officer
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