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Biohub · 1 day ago

Postdoctoral Fellow, Functional Genomics & Epigenomics

New York, NY

Full-time

Onsite

Mid, Senior Level

$93K/yr - $93K/yr

Biohub is leading the new era of AI-powered biology to cure or prevent disease through its 501c3 medical research organization, with the support of the Chan Zuckerberg Initiative. The successful candidate will lead pioneering research into the functional mapping and engineering of non-coding regulatory elements that govern immune cell gene expression, focusing on identifying and manipulating enhancers and other regulatory elements.

BioinformaticsBiotechnologyGenetics

Responsibilities

Apply high-throughput CRISPR/Cas9 and CRISPRi/a screens, STARR-seq, and massively parallel reporter assays (MPRA) to functionally map immune-cell-specific enhancers, silencers, and boundary elements

Develop and optimize protocols for scRNA-seq, scATAC-seq, scCUT&Tag, and multimodal platforms (e.g., 10x Genomics, Atrandi)

Use bulk and single-cell nascent RNA profiling (PRO-seq, scGRO-seq) to dissect transcriptional kinetics in engineered immune cells

Employ targeted histone modification and DNA methylation tools (e.g., dCas9-p300, dCas9-TET1) to modulate enhancer activity in immune contexts

Isolate, culture, and genetically reprogram primary human and murine immune cells, including T cells, B cells, NK cells, dendritic cells, macrophages, and HSPCs

Design logic-gated circuits and inducible systems for spatially and temporally controlled gene expression

Engineer immune cells within human immune organoids, tumor–immune co-cultures, and 3D hydrogel scaffolds

Apply engineered cells in syngeneic and patient-derived xenograft (PDX) mouse models

Partner with teams in immunology, cancer biology, and systems genomics

Publish findings in preprints and peer-reviewed journals, and present at scientific conferences

Support grant development, technology transfer, and mentoring of graduate students and junior researchers

Qualification

CRISPR/Cas genome editingFunctional genomicsSingle-cell genomicsImmune cell biologySynthetic biology circuitsGene regulatory network modelingTroubleshootingCollaborationIndependent work