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Fred Hutch · 1 day ago

Research Technician II

Seattle, WA

Full-time

Onsite

Entry Level

$27.01/hr - $38.45/hr

2+ years exp

Fred Hutchinson Cancer Center is an independent nonprofit organization focused on cancer treatment and research. The Research Technician II will support the molecular profiling team in the McElrath Lab, assisting with high-throughput B cell receptor sequencing assays and performing various molecular biology techniques.

Hospital & Health Care

No H1B

Responsibilities

Perform molecular biology techniques: isolation of RNA/DNA from human lymphocytes for downstream analysis, gene expression by high density multiplex PCR, RT-qPCR, RNA-Seq, RT-PCR amplification for targeted amplicon sequencing of B cell receptor genes (paired and unpaired), including indexing and library preparation for NGS Illumina platform, antibody cloning and purification, monoclonal antibody production by transient transfection

Perform tissue culture techniques: maintenance of cell lines including frozen inventories, transduction of feeder cells with retroviral and lentiviral vectors, virus neutralizations, cytotoxicity assays, in vitro cell manipulations, including peptide stimulations and co-cultures

RNA and DNA isolation from whole blood, PBMC and tissue, Library preparation and indexing for Illumina sequencing platform

Operate and carry out basic maintenance and QC of laboratory equipment

Perform and/or optimize new assays and specimen processing methods as needed

May prepare stocks of HIV pseudoviruses for downstream neutralization

Prepare and maintain reagents, stocks, and cultures necessary to perform the work

Independently collect and analyze data

Drafting, editing and revising SOPs (standard operating procedures) in support of new experimental designs and laboratory operations

Other duties as directed by supervisor, include conducting literature reviews to assist in determining most suitable methods to be used in research, collect, analyze and assist in preparation of data for oral and written presentations

Qualification

Molecular biology techniquesPCRNGS library preparationAseptic tissue cultureData collectionStatistical analysisLaboratory record keepingAttention to detailTeam playerSelf-motivated

Required

Bachelor's degree in related scientific field or equivalent combination of education and experience

2 years of experience working in a lab outside of school coursework

Preferred

Preference towards experience in molecular biology techniques such as PCR, qPCR, digital droplet PCR, NGS library preparation experience, aseptic tissue culture techniques, bacterial cloning and protein expression systems, neutralization assays, ELISAs

Great attention to details, well-organized, self-motivated, and able to efficiently manage time and experiments

Have scientific curiosity, a high level of self-motivation, the ability to take instruction and correction and to follow directions, good organizational skills, the ability to work and think independently, but also someone who is a team player

Show initiative and be willing to take up new laboratory skills and communicate well with other members of the research team

Strong skills in data collection and laboratory record keeping

Computer skills and experience with statistical analysis and data presentation are desirable

Willing to work with biohazardous/infectious agents (e.g., bloodborne pathogens, including HIV-1) under carefully controlled conditions and the flexibility to work some evenings and weekends