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Inside Higher Ed · 1 week ago

Research Specialist (Molecular & Comparative Pathobiology)

Baltimore, MD

Full-time

Onsite

Entry Level

$17/hr - $29/hr

1+ years exp

Johns Hopkins University is seeking a Research Specialist with a strong foundation in advanced research techniques. The role involves managing daily research projects, planning experiments, and evaluating test results while ensuring adherence to laboratory protocols and safety standards.

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Responsibilities

Performs cell culture and processes biofluids and tissues from human donors and animal models. Performs fast protein liquid chromatography (FPLC)-based size separation to obtain proteins and protein complexes, confirming identity of selected isolated proteins. Isolates RNA, performs quality control, and prepares RNA sequencing libraries using methods including advanced, ligation-free preparations. Separation and characterization of extracellular vesicles. Assists with analysis and graphical representation of RNA-seq data. Troubleshoots and optimizes techniques based on scientific knowledge and research experience. Liaises with bioinformatics collaborators

Follows established procedures or protocols and utilizes aseptic and RNase-free technique to perform laboratory tests and/or experiments which include conventional and real-time PCR, electrophoretic separations (agarose and acrylamide gels), Western blot, ELISA, cloning and/or subcloning, extracellular vesicle isolation and characterization, electron microscopy, nanoparticle tracking analysis, resistive pulse sensing, flow cytometry, and other molecular and cellular biology techniques

Learn and train in additional procedures in order to expand skills, foster individual growth, and directly contribute to and facilitate laboratory success

Uses various software applications, such as spreadsheets, relational databases, electronic mail, World Wide Web, statistical packages, and graphics packages to assemble, manipulate and/or format data and/or test reports

Participates in the organization and operation of the laboratory, including oversight of undergraduate student researchers. Performs general lab maintenance duties and maintains excellent written records and electronic databases

Uses universal safety precautions to protect self and co-workers from biohazardous materials, including blood-borne pathogens. Completes annual university biohazard/universal precaution/radiation safety training, as appropriate. Monitors biohazard/radiation safety standards and proper handling of potentially hazardous chemical and biological agents and/or radiation sources in the workplace