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San Diego Supercomputer Center · 1 day ago

Staff Research Associate II - 138072

San Diego, CA

Full-time

Onsite

Lead/Staff

$30.32/hr - $48.78/hr

The San Diego Supercomputer Center is part of UC San Diego Health Sciences, which encompasses a wide range of academic departments and programs. The Staff Research Associate II will perform laboratory procedures with human stem cells and primary cells, contributing to disease model development for drug discovery and supporting various research projects in the Cellular and Molecular Medicine department.

Big DataDatabaseInformation Technology

Comp. & Benefits

Responsibilities

Independently performs standard laboratory procedures with adult human stem cells and primary cells, human pluripotent stem cells (hPSCs), including embryonic stem cells (hESCs) and induced pluripotent stem cells (hiPSCs), as well as other experimental procedures

Uses basic molecular biology and microscopy techniques to independently conduct a number of experimental procedures

Uses microscopes, high content imaging, plate-readers, flow cytometers, cell fractionators, isolators, microfluidic chambers, and available robotics to assay effects of small molecules on stem cells and organoids grown from stem cells will be necessary

Performs standard experimental protocols in tissue culture to meet the needs of the research projects, providing ideas and rationale to help improve tests that are unsatisfactory

Edits and maintains database of human tissue and organoids in the HUMANOID biorepository, maintain up-to-date clinic IRBs (Consent forms and IRB protocols), and serve as runner if needed to secure patient-derived tissues from clinics and bring them to the HUMANOID for organoid isolation

Keeps up with production of conditioned base media for lab and customer use

Performs and keeps record of QC of all new cell lines and media batches

Performs other duties as assigned

Qualification

Molecular biology techniquesMammalian cell cultureMicroscopyDNA techniquesImmunofluorescent labelingStatistical analysisLearn quicklyOrganization skillsAttention to detailCommunication skills

Required

Theoretical knowledge of molecular and cellular biology or closely related field as typically acquired at a collegiate level or equivalent combination of education and experience

Working knowledge of DNA techniques

Lab experience, knowledge and understanding of sterile techniques and mammalian cell culture practices

Knowledge and experience with numerous types of laboratory equipment related to uses, maintenance, and repair

Ability to learn new laboratory procedures quickly

Ability to prepare solutions

Strong organization skills

Attention to detail; self motivated

Ability to learn quickly and adopt lessons from one learning episode to similar situations

Ability to perform a variety of tasks in an extremely fast-paced environment

Use of good judgement relating to constantly changing priorities

Ability to work as part of a large, diverse group

Knowledge of various computer software, hardware, standard office equipment and lab equipment

Acute attention to detail, resourceful, capable of handling the changing needs of the research lab

Experience performing repetitive and specialized laboratory experimental procedures

Experience following experimental protocols

Ability to care for small lab animals, as well as working with animal and human tissue

Ability to use mathematical skills to analyze and develop statistical data

Strong communication skills (both verbal and written) needed to interact professionally and effectively in the work environment at all levels including faculty, senior researchers, graduate students, undergraduates and those from various backgrounds with tact and diplomacy

Experience using microscopy to perform experiments

Experience with mammalian tissue culture

Experience with immunofluorescent labeling, microscopy, other plate-based assays, and small molecule library screens