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San Diego Supercomputer Center · 1 week ago

Laboratory Assistant - 138039

San Diego, CA

Contract

Onsite

New Grad, Entry Level

$27.85/hr - $33.22/hr

The San Diego Supercomputer Center is part of UC San Diego and is dedicated to advancing cancer research and patient care. The Laboratory Assistant will support research projects focused on pancreatic cancer by performing various laboratory techniques, managing mouse colonies, and assisting with data preparation for publication.

Big DataDatabaseInformation Technology

Comp. & Benefits

Responsibilities

Assist in characterizing the impact of the germline BRCA2 mutation on pancreatic cancer initiation and progression through preclinical studies using GEMMs

Support research by processing PDAC patient blood samples to isolate DNA for long-read sequencing and PBMCs for immunological and biological analyses

Contribute to recombinant DNA work and genome engineering

Perform a variety of cell culture techniques, including flow cytometry for cell surface protein expression, cell propagation, contaminant testing, and cryopreservation

Assist with mouse colony management and genotyping using established PCR techniques

Work with small animals and assist with procedures such as surgery, pancreatic orthotopic injections, ultrasound imaging, and pregnancy and tumor monitoring

Assist with immunohistochemistry of frozen and fixed tissues or cells

Assist with protein biochemistry techniques, including protein quantification, SDS PAGE, immunoprecipitation, and Western blotting

Maintain and prepare accurate records of all experiments

Assist in the preparation of data for publication

Other duties assigned as needed

Qualification

Molecular biologyCell culture techniquesPCR techniquesImmunohistochemistryProtein biochemistryAnimal handlingEnthusiasm for learningBasic computer applicationsCommunication skillsOrganizational skillsDetail-oriented

Required

Theoretical knowledge of molecular biology, immunology, cell biology, and/or biochemistry

Ability to perform blood sample preparations including centrifugation, pipetting, making various buffer solutions, and proper sample storage. Ability to use biosafety cabinet, ULT/LN freezers, and centrifuge

Ability and willingness to handle and work with small animals. Ability to perform mouse genotyping using DNA isolation, PCR-based methods and agarose gel electrophoresis, and basic molecular biology experiments. Ability to assist in performing mouse surgeries, experiments, and injections

Ability to perform basic DNA and protein characterization techniques using electrophoretic analysis, quantification using plate-based analysis, and immunoblot-based analysis

Strong communication skills (both verbal and written) needed to interact professionally and effectively in the work environment, as well as to effectively and accurately document research procedures. Ability to read, comprehend and discuss research materials

Ability to maintain confidentiality. Organizational skills to order and maintain lab equipment and supplies

Basic understanding of computer applications such as Word, Excel and PowerPoint. Internet search and software analysis capabilities are preferred

Good organizational skills and ability to prioritize work

Enthusiasm for learning and a genuine interest in scientific research. Willingness and ability to learn new skills, research methods, and scientific principles

Familiarity with basic laboratory equipment such as centrifuges, spectrophotometers, balances, pH meters, autoclaves and electrophoresis equipment

Ability to adhere to established UCSD guidelines for safety and environmental issues

Familiar in proper handling of chemical and biohazard materials. Familiarity with sterile techniques

Detail-oriented. Ability to work with little supervision. Ability to follow verbal and/or written instructions. Ability to follow-up on previously begun procedures. Ability to accurately record data and enter into appropriate medium