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10x Genomics · 19 hours ago

Molecular Biology Intern (High-Throughput Assays)

Pleasanton, CA

Internship

Onsite

Intern

$40/hr - $48/hr

10x Genomics is dedicated to accelerating our understanding of biology, and they are seeking a Molecular Biology Intern to join their spatial proteomics R&D effort. The intern will focus on building and optimizing high-throughput experimental workflows for screening custom proteins, while collaborating with a cross-functional team in molecular biology, protein engineering, and computational design.

Biotechnology

H1B Sponsor Likely

Responsibilities

Develop, optimize, and execute high-throughput screening assays to evaluate custom proteins

Build and refine yeast surface display workflows (and/or related display platforms) for quantitative binder characterization using flow cytometry

Establish and compare surface-based screening formats, such as:

Yeast display + FACS

Surface-immobilized target assays (e.g., beads, chips, slides)

Plate-based or bead-based binding and competition assays

Implement and benchmark conjugation strategies that link proteins to DNA or other reporter molecules for multiplexed assays and barcoded readouts

Design and execute cloning strategies for custom protein libraries and individual variants (PCR, Gibson/Golden Gate, site-directed mutagenesis)

Construct and validate expression vectors for yeast and/or other systems, including QC of expression and display levels

Support expression and basic QC of selected binders (e.g., SDS-PAGE, Western blot, simple purification or lysate-based functional assays)

Plan and perform FACS-based and/or plate-based binder screening experiments, including appropriate controls, titrations, and replicates

Analyze high-dimensional assay data (e.g., from flow cytometry or barcoded readouts) to rank candidates based on affinity, specificity, and other performance metrics

Where applicable, prepare samples and data for sequencing-based readouts and collaborate with others to interpret sequence–function relationships

Summarize findings in clear figures, tables, and internal reports, and present progress in regular team meetings

Work with computational design, protein production, and assay development teams to integrate experimental results into the design–build–test–learn cycle

Maintain detailed electronic lab notebook records, SOPs, and reagent documentation to ensure reproducibility and handoff

Contribute to a final internal report and presentation describing the developed workflows, experimental results, and recommendations for future development

Qualification

Molecular BiologyHigh-Throughput ScreeningProtein EngineeringFlow CytometryData AnalysisCloning TechniquesOrganizationClear CommunicationCollaboration

Required

Current PhD candidate in Biochemistry, Biophysics, Molecular Biology, Bioengineering, Chemical Biology, or a closely related field

Ideally within the final 1–2 years of their PhD program, with substantial hands-on experimental training in protein or molecular biology

Proven ability to independently design, execute, and troubleshoot experiments

Strong quantitative and analytical mindset, including comfort with large datasets from high-throughput screens

Clear written and verbal communication skills, with the ability to present complex results across disciplines

Highly organized, collaborative, and comfortable working in a fast-paced, interdisciplinary R&D environment

Strong molecular biology foundation: PCR, cloning (e.g., Gibson, Golden Gate), site-directed mutagenesis

Experience with protein expression and characterization in at least one system (yeast, E. coli, or cell-free): Induction/optimization of expression, basic purification or lysate-based assays, SDS-PAGE/Western

Hands-on experience with high-throughput or quantitative assay formats, such as: Flow cytometry / FACS, plate-based binding assays (ELISA or similar), or cell-based functional assays

Comfort with data analysis and visualization

Preferred

Experience with Python, R, or analysis tools such as GraphPad Prism is nice to have

General awareness and experience with AI data analysis

Yeast surface display, phage display, ribosome/mRNA display, or other library-based screening technologies

Experience with conjugation and bioconjugation methods, for example: Cysteine-based conjugation (maleimide-thiol, halo tag, SNAP/CLIP, etc.), Click chemistry (e.g., azide–alkyne, DBCO, strain-promoted cycloaddition), DNA-barcoding of proteins, or other methods linking proteins to nucleic acids or reporters

Familiarity with translation-linked or puromycin-based conjugation methods, or related approaches that couple protein synthesis to covalent capture is highly desired

Background in protein engineering, antibody/nanobody development, or de novo mini-binder workflows

Company

10x Genomics

Glassdoor3.5

Cells are the basic unit of life.

Founded in 2012

Pleasanton, California, US

1001-5000 employees

http://10xgenomics.com

H1B Sponsorship

10x Genomics has a track record of offering H1B sponsorships. Please note that this does not guarantee sponsorship for this specific role. Below presents additional info for your reference. (Data Powered by US Department of Labor)

Distribution of Different Job Fields Receiving Sponsorship

Represents job field similar to this job

Trends of Total Sponsorships

2025 (41)

2024 (40)

2023 (35)

2022 (52)

2021 (34)

2020 (16)