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University of Pittsburgh · 2 months ago

Laboratory Research Technician IV

Pittsburgh, PA

Full-time

Onsite

Mid Level

3+ years exp

The University of Pittsburgh is seeking a Laboratory Research Technician IV to join their translational research team in the Khan Lab, which investigates mechanisms linking inflammation and carcinogenesis in colorectal cancer. The role involves performing complex laboratory work, managing workflows, and supporting data analysis and manuscript writing.

Higher Education

No H1B

Responsibilities

Perform and oversee molecular and cellular experiments, including DNA/RNA extraction, PCR, Western blotting, and ELISA

Conduct murine studies including genotyping, sample collection (colon, lymphoid organs), tissue processing, and histology preparation (FFPE, frozen)

Establish and maintain primary epithelial and immune cell cultures, including murine and human colonic organoids, and perform co-culture and drug treatment assays

Execute and assist with immunofluorescence and immunohistochemistry

Assist in flow cytometry and single-cell RNA sequencing sample preparation

Maintain detailed electronic lab notebooks and organize experimental data for analysis and publication

Support the PI and trainees in data analysis, figure preparation, and manuscript writing

Manage laboratory inventory, ordering, and compliance with safety protocols; serve as lab safety liaison as needed

Train and mentor junior staff, students, or fellows in lab techniques

Qualification

Molecular biologyHistologyCell-based assaysDNA/RNA extractionPCRWestern blottingELISAFlow cytometrySingle-cell RNA sequencingData analysisManuscript writingTrainingLaboratory inventory managementMentoring

Required

Bachelor's Degree

3 years of experience

Experience in molecular biology, histology, and cell-based assays

Ability to manage complex workflows independently

Comfortable in both mouse and human tissue research settings