Legend Biotech · 10 hours ago
Molecular Biology Scientist/Senior Associate Scientist
Philadelphia, PA
Full-time
Onsite
Mid, Senior Level
$93K/yr - $123K/yr
2+ years expLegend Biotech is a global biotechnology company dedicated to treating and curing life-threatening diseases. They are seeking a Molecular Biology Scientist/Senior Associate Scientist to support lentiviral vector design, construction, and functional evaluation, as well as engineered cell line development for CAR-T and other cell therapy programs.
BiotechnologyHealth CareLife ScienceOncologyTherapeutics
Responsibilities
Execute viral vector plasmid and helper plasmid design and construction to support gene overexpression, knock-down, and knock-out studies (under guidance for Senior Associate Scientist; independently for Scientist)
Perform routine cloning workflows and confirm constructs via restriction digest and sequencing analysis
Maintain accurate plasmid records including sequence files, maps, inventory/locations and support internal construct requests in partnership with other cross-functional teams
Generate and characterize engineered mammalian cell lines for stable vector production, single-cell cloning, cell engineering and basic characterization
Perform mammalian cell culture and transient transfection under BSL-2 conditions
Support small-scale vector production using suspension cell lines for early development activities
Execute molecular/cellular assays to evaluate vector performance using qPCR/ddPCR, ELISA/Western Blot, flow cytometry
Analyze data, summarize results, and present clear experiment conclusions, escalate issues and propose next steps to project leads
Adhere to BSL-2 biosafety standards and Legend policies, maintaining accurate and compliant electronic lab notebooks and documentation
Collaborate cross-functionally to support tech transfer activities as needed
Works from established protocols, identifies deviations and escalates promptly
Independently plans day-to-day execution within project goals, recommends optimizations and next experiments
Qualification
Required
Associate Scientist: B.S. with 2+ years or M.S. with 0-2 years in Molecular Biology, Virology, Cell & Gene Therapy, Biochemistry, or related field
Scientist: M.S. with 3+ years or Ph.D. with 0-2 years in a related field; hands-on molecular cloning and mammalian cell culture required
Hands-on experience in molecular biology techniques (cloning, plasmid prep, sequence analysis, construct verification)
Experience with mammalian cell culture and transient transfection; familiarity with BSL-2 practices and documentation
Experience with titering and functional readouts (e.g., transduction efficiency, gene expression)
Experience with qPCR/ddPCR, flow cytometry, Western blot and ELISA for tittering and functional assays
Strong ELN documentation, sample tracking, and ability to follow protocols/SOPs with attention to detail
Adaptable to a fast-paced environment; effective prioritization, collaboration, and communication
CRISPR/Cas gene editing for KO/KI or multiplexed cell lines
Familiarity with viral vector process development concepts (upstream and/or downstream)
Exposure to NGS for vector or genome characterization
Organized and detail-oriented; able to manage multiple experiments/projects
Analytical and problem-solving skills to troubleshoot and implement improvements
Collaborative; able to work effectively within a team environment
Maintain accurate ELN, sample tracking, and inventory records
Clear written and verbal communication for documentation and data presentation
Preferred
Experience generating engineered cell lines (stable expression and/or CRISPR KO/KI) including cloning and basic screening (Scientist preferred)
Exposure to viral vector production and/or viral vector workflows (LVV preferred)
Tech transfer and/or CMC documentation experience (nice to have)
Benefits
Medical, dental, and vision insurance
401(k)-retirement plan with company match that vest fully on day one
Equity and stock options
Eight weeks of paid parental leave after just three months of employment
Paid time off policy that includes vacation days, personal days, sick time, 11 company holidays, and 3 floating holidays
Flexible spending and health savings accounts
Life and AD&D insurance
Short- and long-term disability coverage
Legal assistance
Supplemental plans such as pet, critical illness, accident, and hospital indemnity insurance
Commuter benefits
Family planning and care resources
Well-being initiatives
Peer-to-peer recognition programs
Company
Legend Biotech
Legend Biotech is a biopharmaceutical company that develops and commercializes novel cell therapies for oncology.
1001-5000 employees
H1B Sponsorship
Legend Biotech has a track record of offering H1B sponsorships. Please note that this does not
guarantee sponsorship for this specific role. Below presents additional info for your
reference. (Data Powered by US Department of Labor)
Distribution of Different Job Fields Receiving Sponsorship
Represents job field similar to this job
Trends of Total Sponsorships
2025 (30)
2024 (11)
2023 (14)
2022 (7)
2021 (6)
2020 (13)
Funding
Current Stage
Public CompanyTotal Funding
$750.45M2023-05-05Post Ipo Equity· $350M
2022-07-25Post Ipo Equity· $250M
2020-06-05IPO
Leadership Team
Carlos Santos
Chief Financial Officer
Alan Bash
President, CARVYKTI
Recent News
2025-12-18
Company data provided by crunchbase