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GAP Solutions, Inc. · 15 hours ago

Scientist

Bethesda, MD

Full-time

Onsite

Mid Level

GAP Solutions, Inc. is seeking a Scientist to support the Laboratory of Malaria and Vector Research within the National Institute of Allergy and Infectious Diseases. The role involves performing advanced cellular and molecular analyses, including genetic manipulation and characterization of vascular cells, as well as contributing to research projects and maintaining laboratory operations.

Management Consulting

H1B Sponsor Likely

Responsibilities

Perform microdissections of human vessels, preparing tissues for single cell analysis of vascular cells, performing genetic manipulation of human vessels, and characterizing their function using molecular, imaging, and physiologic evaluations

Perform cell culture of vascular cells, preparing cells for single cell analysis, performing genetic manipulation of cultured cells, and characterizing their function using molecular, imaging, and physiologic evaluations

Perform phenotyping of vascular function in mouse models of human diseases or conditions

Characterize protein-protein interactions involved in vascular cell signaling and pharmacologic targeting of these pathways to modify vascular function

Develop, optimize, and conduct microdissections of human blood vessels from tissue specimens as part of IRB-approved or IRB-exempt studies

Develop, optimize, and conduct culture and co-culture of vascular cells

Develop or optimize approaches to study human vessels and/or cultured cells at the single cell level as part of IRB-approved or IRB-exempt studies

Perform genetic manipulation of human blood vessels ex vivo or cultured cells in vitro to express, silence, or modify genes of interest

Develop and apply molecular tools for confirming successful gene editing or inactivation

Characterize human vascular function ex vivo using myography, imaging, gene expression, and/or molecular sensors as part of IRB-approved or IRB-exempt studies

Characterize cultured cells using imaging, gene expression, and/or molecular sensors

Perform vascular phenotyping of rodent models, including mouse models of human diseases or conditions

Perform biochemical characterization of protein-protein interactions involved in vascular cell signaling

Develop and test pharmacologic approaches to target protein-protein interactions to modify vascular function in human vessels or in cell or animal models of vascular physiology or disease

Keep and maintain a clean and efficient working laboratory

Order and organize necessary supplies and reagents

Maintain laboratory notebooks with sufficient details for reproducing results and documenting progress to support intellectual property evaluations

Maintain laboratory safety

Maintain accurate sample tracking and data management

Perform problem solving, call technical support to fix problems, initiate service calls or repairs, and interact with field service engineers and field application specialists for repair and optimization of laboratory equipment

Advise and assist the Federal Task Leader and other lab staff in molecular physiologic techniques

Assist and perform lab projects independently

Discuss and report data in peer reviewed papers and with the Federal Task Leader and scientists

Qualification

Genetic manipulationSingle cell analysisMolecular characterizationCell cultureVascular phenotypingLaboratory safetyData managementProblem solving

Required