Saviance · 15 hours ago
Scientist 2, Biology
San Diego, CA
Full-time
Onsite
Entry, Mid Level
2+ years expSaviance is seeking a highly motivated and organized Scientist II to contribute to the research and development of monoclonal antibodies in their Antibody Technologies team. The individual will be responsible for executing experiments in protein expression and purification, tissue culture, and testing antibodies, requiring extensive hands-on experience in these areas.
Information Technology & Services
Responsibilities
Responsible for purification of antibodies and recombinant proteins using AKTA FPLC and manual methods. Must execute and document work using SOPs and must follow strict GMP requirements under ISO 13485 standards. Coordinates preparation and transfer of purified antibody and protein samples to other functional teams
Executes analytical testing of purified antibodies and proteins using SEC, SDS-PAGE, Western blot, MADLS, and qPCR
Responsible for transfection and/or expression of antibodies and recombinant proteins from various mammalian cell lines, including but not limited to CHO and hybridomas as well as *E. coli*. Specific requirement for hands-on experience with aseptic technique and cell-culture techniques
Responsible for the execution of experiments with varying complexity using advanced molecular biology techniques
Responsible for the execution of experiments with varying complexity using multi-color Flow Cytometry
Analyze data using FCS Express and FlowJo software, report test results including completion of all required documentation and database entries as required by protocols and SOPs
Day-to-day problem solving and cross-functional team-based activities to ensure that daily operations and timelines are met
Independently analyze own research data, solve problems with minimal guidance and effectively plan follow up experiments. Will be expected to efficiently prioritize individual workload
Involvement with scheduling, operational and maintenance duties required within the laboratory
Qualification
Required
A Bachelor's or Master of Science degree in biochemistry, cellular or molecular biology, immunology, or related discipline and a minimum of 2-4 years of laboratory experience required
Protein purification, protein expression, molecular biology and tissue culture experience required
Responsible for purification of antibodies and recombinant proteins using AKTA FPLC and manual methods
Must execute and document work using SOPs and must follow strict GMP requirements under ISO 13485 standards
Coordinates preparation and transfer of purified antibody and protein samples to other functional teams
Executes analytical testing of purified antibodies and proteins using SEC, SDS-PAGE, Western blot, MADLS, and qPCR
Responsible for transfection and/or expression of antibodies and recombinant proteins from various mammalian cell lines, including but not limited to CHO and hybridomas as well as E. coli
Specific requirement for hands-on experience with aseptic technique and cell-culture techniques
Responsible for the execution of experiments with varying complexity using advanced molecular biology techniques
Responsible for the execution of experiments with varying complexity using multi-color Flow Cytometry
Analyze data using FCS Express and FlowJo software, report test results including completion of all required documentation and database entries as required by protocols and SOPs
Day-to-day problem solving and cross-functional team-based activities to ensure that daily operations and timelines are met
Independently analyze own research data, solve problems with minimal guidance and effectively plan follow up experiments
Will be expected to efficiently prioritize individual workload
Involvement with scheduling, operational and maintenance duties required within the laboratory
Effective interpersonal skills and ability to work in a cross-functional team-oriented environment to achieve organizational goals
Effective written and oral communication skills
Demonstrated ability to follow established policies and procedures
Demonstrated ability to keep neat, accurate, and complete records
Effective analytical and problem-solving skills
Proficient with aseptic technique and cell-culture techniques is required
Protein expression using various systems including but not limited to mammalian cells, insect cells, and bacteria
Protein purification with liquid chromatography and quality assurance using various analytical methods
Preferred
Familiarity with assay automation and automated liquid handlers
Experience with cell line engineering
Hand-on experience with Flow Cytometry and the ability of analyzing Flow data is preferred
Company
Saviance
Saviance is a modern consulting firm providing a variety of professional services to its clients in the US.
201-500 employees
Funding
Current Stage
Growth StageLeadership Team
Rohit M.
Managing Partner
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