Mass General Brigham · 4 hours ago
Research Technician II
Charlestown-MA
Full-time
Onsite
Entry, Mid Level
$24.28/hr - $39.43/hr
2+ years expMass General Brigham is a not-for-profit organization that supports patient care, research, teaching, and community service. They are seeking a Research Technician II to work independently under supervision in the lab of Dr. Christopher Mount, generating and evaluating cellular immunotherapies and performing molecular characterization assays.
Health CareHome Health CareMedical
Responsibilities
Generate and maintain CAR-T cells, including production of viral stocks, collection of primary T cells, cell transduction and expansion, and maintenance of associated records
Cell culture of diverse human cell lines, including brain tumor models and patient-derived organoids
Assay the activity of CAR-T cells *in vitro*, including cytokine generation, cytotoxicity, and cellular imaging
Flow cytometric analysis of CAR-T cells
Molecular cloning, including coordination and assessment of sequencing results and maintenance of documented records of constructs
Execution of protocol-based genomics assays including single cell sequencing, spatial transcriptomics, and spatial proteomics
Interface with collaborators and service core providers to transfer specimens, submit service requests, and oversee receipt of data and services. This may involve travel to local MGB hospitals to collect patient tissue specimens (shuttles available at no cost)
Basic laboratory management including inventory management, package receipt/shipping, routine instrument monitoring and maintenance, maintaining and distributing key lab protocols
This role requires being listed as an emergency contact alongside other team members for mission-critical instrumentation (such as scientific freezers). In the unexpected event that such instrumentation fails, this may result in after-hours contact and a responsibility to help coordinate a response alongside other team members
Attendance and presentation at weekly lab scientific meetings
Qualification
Required
A documented record of at least two years (post-undergraduate) in an academic or industry laboratory setting
Generate and maintain CAR-T cells, including production of viral stocks, collection of primary T cells, cell transduction and expansion, and maintenance of associated records
Cell culture of diverse human cell lines, including brain tumor models and patient-derived organoids
Assay the activity of CAR-T cells in vitro, including cytokine generation, cytotoxicity, and cellular imaging
Flow cytometric analysis of CAR-T cells
Molecular cloning, including coordination and assessment of sequencing results and maintenance of documented records of constructs
Execution of protocol-based genomics assays including single cell sequencing, spatial transcriptomics, and spatial proteomics
Interface with collaborators and service core providers to transfer specimens, submit service requests, and oversee receipt of data and services
Basic laboratory management including inventory management, package receipt/shipping, routine instrument monitoring and maintenance, maintaining and distributing key lab protocols
This role requires being listed as an emergency contact alongside other team members for mission-critical instrumentation
Attendance and presentation at weekly lab scientific meetings
An ability to clearly articulate one's role(s) in this past experience, including scientific question or goal, and how one's work contributed to the efforts of the larger team
Enthusiasm, eagerness to learn and develop as part of a collaborative team
Ability to work independently and rigorously document one's work
Excellent written and verbal communication skills
Strong organizational skills and attention to detail
Preferred
Experience with CAR-T cell production and relevant in vitro assays
Experience with molecular cloning, including validation of generated constructs
Prior mammalian cell culture work, including lentiviral production
Previous work performing single cell sequencing, spatial transcriptomics, or immunofluorescence microscopy
Experience with histology, including specimen preparation, cryosectioning or FFPE sectioning, and immunostaining
Software: Microsoft suite including Excel, Graphpad Prism, Flowjo, Snapgene or other molecular cloning software
Benefits
Comprehensive benefits
Career advancement opportunities
Differentials
Premiums and bonuses
Recognition programs designed to celebrate your contributions and support your professional growth
Company
Mass General Brigham
Mass General Brigham specializes in providing medical treatments and health diagnostics services.
10001+ employees
H1B Sponsorship
Mass General Brigham has a track record of offering H1B sponsorships. Please note that this does not
guarantee sponsorship for this specific role. Below presents additional info for your
reference. (Data Powered by US Department of Labor)
Distribution of Different Job Fields Receiving Sponsorship
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Trends of Total Sponsorships
2025 (77)
2024 (61)
2023 (93)
2022 (70)
2021 (80)
2020 (29)
Funding
Current Stage
Late StageLeadership Team
E
Erin Flanigan
SVP, Human Resources, Community, Specialty Hospital Division
O’Neil A. Britton
Chief Integration Officer, Executive Vice President
Recent News
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