Bayside Solutions · 22 hours ago
Scientist
Berkeley, CA
Contract
Onsite
Entry, Mid Level
2+ years expBayside Solutions is looking for a Scientist to execute and optimize high-stakes cell culture, molecular biology, and long-read sequencing workflows. The ideal candidate will play a pivotal role in therapeutic screening and platform advancement, translating high-level experimental goals into reproducible workflows.
Information TechnologyStaffing AgencyTelecommunicationsVirtual Reality
No H1B
Responsibilities
Execute and optimize cell culture and electroporation workflows, maintaining primary and engineered systems to support therapeutic screening
Design and run ddPCR assays as primary readouts, owning everything from primer design to data analysis of knockdown efficiency
Prepare high-molecular-weight DNA and intact RNA for PacBio Revio HiFi and Iso-Seq workflows
Execute SMRTbell library prep, including end repair, adapter ligation, and size selection for optimal Revio platform performance
Perform quality control using Qubit, NanoDrop, TapeStation/Femto Pulse, and fragment analysis
Partner with internal teams and vendors to resolve issues like low yield or suboptimal read lengths; support the transition to automated liquid-handling systems
Establish robust SOPs and support the scale-up of molecular assays
Qualification
Required
B.S. in Molecular Biology, Cell Biology, Bioengineering, or a related field (Advanced degree preferred)
2+ years of hands-on experience with core techniques (nucleic acid extraction, PCR/qPCR/ddPCR, cloning)
1+ year of experience with aseptic technique for mammalian tissue culture
1+ year of experience in NGS sample prep, library preparation, and associated quality control
Direct experience culturing and differentiating human ES cells and/or iPS cells is required
Ability to work independently in a fast-paced environment and clearly communicate technical blockers and progress
Preferred
Cell culture
mammalian tissue culture
aseptic technique
primary and engineered cell systems
human ES cell culture
iPS cell culture
cell differentiation
electroporation
therapeutic screening assays
molecular biology
nucleic acid extraction
high-molecular-weight DNA preparation
RNA isolation
PCR
qPCR
ddPCR assay design
primer design
knockdown efficiency analysis
cloning
NGS sample preparation
long-read sequencing workflows
PacBio Revio HiFi sequencing
Iso-Seq
SMRTbell library preparation
end repair
adapter ligation
size selection
quality control (Qubit, NanoDrop, TapeStation, Femto Pulse, fragment analysis)
data-driven experimental optimization
SOP development
assay scale-up
troubleshooting yield and read-length issues
collaboration with cross-functional teams and vendors
automation and liquid-handling systems
independent experimental execution
technical communication
Company
Bayside Solutions
Bayside Solutions is a staffing and recruiting company offering IT, telecom, and scientific staffing services.
51-200 employees
Funding
Current Stage
Growth StageLeadership Team
B
Bob Klotz
Founder
Company data provided by crunchbase