e184 · 8 hours ago
Associate Scientist, Cell Biology
Portland, Oregon
Full-time
Onsite
Mid Level
3+ years expe184 is a biotechnology research company advancing in vitro gametogenesis to transform reproductive medicine. They are seeking a highly skilled Associate Scientist to execute critical experimental workflows that support high-throughput screening and cell engineering programs.
Biotechnology
Responsibilities
Maintain and characterize pluripotent stem cell lines and primary cells, including routine culture, differentiation, quality control testing, cryopreservation, and cell banking according to established protocols
Design and implement genome engineering strategies using CRISPR/Cas systems to generate reporter lines and other genetic modifications supporting screening and validation experiments
Generate and validate engineered cell lines, including screening clones for correct genomic integration, characterizing expression patterns, and establishing stable reporter systems for phenotypic assays
Execute genetic screening experiments in both pooled and arrayed formats, managing experimental workflows from library transduction through sample collection and quality control
Optimize culture conditions and cellular assay parameters to ensure robust experimental performance, troubleshoot technical challenges, and provide rotational weekend maintenance when required
Perform molecular biology workflows including plasmid preparation and library construction to support screening and validation campaigns
Prepare samples for genomic analyses including single-cell RNA sequencing and other next-generation sequencing applications, ensuring sample quality and proper experimental controls
Conduct flow cytometry and immunofluorescence analyses to characterize cellular phenotypes, validate reporter systems, and support data collection for screening campaigns
Qualification
Required
BS in Biology, Molecular Biology, Cell Biology, Biochemistry, or related field with 4+ years of relevant experience, OR MS in related field with 3+ years of relevant experience
Hands-on mammalian cell culture experience with sterile technique
Demonstrated proficiency with molecular biology techniques (PCR, gel electrophoresis, plasmid preparation)
Experience maintaining cell lines (passaging, cryopreservation, quality control)
Basic flow cytometry experience (sample preparation and data collection)
Ability to follow protocols accurately and troubleshoot basic issues
Proficient with laboratory equipment and instrumentation
Strong record-keeping and documentation practices
Reliable and responsible for time-sensitive culture maintenance
Willingness to learn new techniques and adapt protocols
Flexibility for occasional weekend cell culture maintenance
Preferred
Direct experience with pluripotent stem cells (iPSCs, ESCs)
Hands-on experience with CRISPR/Cas9 genome editing
Involvement in genetic screening projects (pooled or arrayed)
Experience with reporter cell line generation and validation
Clone screening and characterization experience
Advanced flow cytometry (panel design, multi-color analysis, cell sorting)
Transposon-based genome engineering (PiggyBac, Sleeping Beauty, etc.)
Immunofluorescence microscopy and imaging
Sample preparation for next-generation sequencing (RNA-seq, single-cell sequencing)
Experience with FlowJo, Prism, or similar analysis software
Familiarity with automated liquid handling or high-throughput platforms
Benefits
Medical
Dental
Vision
401(k) with employer match
Company
e184
e184 is an Advanced Research Funding Organization (ARFO) that supports frontier science and technology.
2-10 employees
Funding
Current Stage
Early StageCompany data provided by crunchbase