UC San Diego Health · 6 hours ago
Stem Cell Research Technician - 138387
UC San Diego Health is a leading organization in health sciences, and they are seeking a Stem Cell Research Technician to assist with generating and analyzing stem-cell based models of neurodevelopmental disorders. The role involves performing routine maintenance of stem cells, conducting molecular biology techniques, and assisting with lab organization procedures under supervision.
Health Care
Responsibilities
Assist with generating and analyzing stem-cell based models of neurodevelopmental disorders
Perform routine maintenance of human embryonic and induced pluripotent stem cells and their differentiated progeny, using established protocols
CRISPR genome editing and routine differentiation of these cells towards neuronal cells, using established protocols
Use standard molecular biology tools to transfect, lentivirally infect and electroporate cells, isolate RNA, DNA and protein from cells, and characterize gene expression by Western blot, rt-qPCR, and through the preparation of RNA-seq libraries, using standard kits and procedures
Assist in immunocytochemistry and epifluorescence and confocal microscopy characterization of cells
Other tasks as assigned by the supervisor and project scientists
Involved with general lab organization procedures
Qualification
Required
Theoretical and/or practical knowledge in cellular and molecular biology, immunology, stem cell or related field
Previous experience in human pluripotent stem cell culture and differentiation
Ability to perform molecular biology techniques including PCR, DNA preparation/extraction, gel electrophoresis
Capable of performing biochemistry and laboratory techniques and demonstrated experience to prepare buffers and solutions, as well as various staining solutions
Experience with the operation of a personal computer and software application, such as Access, MS Word and Excel
Ability to work with confocal, light, and fluorescence microscopes
Ability to follow step based experimental protocols
Ability to maintain effective working relationships with other staff members, students, faculty and scientists in a multi-cultural lab
Experience with general lab maintenance including cleaning, stocking supplies
Ability to prioritize and perform efficiently
Willingness to comply to UCSD policies and procedures and to stay informed and comply with basic safety rules and issues
Skill and ability to keep accurate data
Ability to read, comprehend, and discuss research materials
Demonstrated ability to follow established Safety and Biohazardous Material Guidelines
Company
UC San Diego Health
UC San Diego Health is the only academic health system in the San Diego region.
H1B Sponsorship
UC San Diego Health has a track record of offering H1B sponsorships. Please note that this does not
guarantee sponsorship for this specific role. Below presents additional info for your
reference. (Data Powered by US Department of Labor)
Distribution of Different Job Fields Receiving Sponsorship
Represents job field similar to this job
Trends of Total Sponsorships
2025 (308)
2024 (261)
2023 (232)
2022 (223)
2021 (131)
2020 (105)
Funding
Current Stage
Late StageTotal Funding
$0.2MKey Investors
The Beryl InstituteThe Conrad Prebys Foundation
2025-10-31Grant
2023-06-26Grant· $0.2M
Recent News
2025-11-08
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