66 applicants

Company

Original Job Post

Poseida Therapeutics, Inc. · 2 days ago

Senior Research Scientist - Genome/Protein Engineer

San Diego, CA

Full-time

Onsite

Senior Level

$98K/yr - $119K/yr

5+ years exp

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BiopharmaBiotechnology

H1B Sponsorship

Comp. & Benefits

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Responsibilities

Expand the proprietary gene editing platform to develop new tools and/or applications for specialized and enhanced implementation in gene therapy, gene editing, and biologics/cell therapeutics

Apply strong background in molecular and cellular biology to develop and/or enhance new gene editing and transposon tools in cell culture models for use in ex vivo and in vivo therapies

Work in a highly collaborative, cross-functional team environment to perform experiments in the lab, analyze results, and communicate findings in team meetings

Keep current on trends, new products and technologies in gene editing, knock-ins, and transposons

Actively participate in the proprietary gene therapy platform technologies to create new human therapeutic candidates

Analyze data from cell-based assays and report to multidisciplinary groups

Manage 1-2 Research Technicians or Research Associates at the Senior Research Scientist level

Possess 5+ years of relevant industry experience at the Senior Research Scientist level

Qualification

Find out how your skills align with this job's requirements. If anything seems off, you can easily click on the tags to select or unselect skills to reflect your actual expertise.

TransposasesRecombinasesCRISPRTALENsZFNsPrime EditingBase EditingGenome editingBioinformaticsMachine learningPythonFunctional assaysGene editingKnock-Knock-outsGenotoxicityDirected evolutionProtein engineeringCloning techniquesRestriction digestsGibson assemblyTransformationsSanger sequencingPlasmid prepsDNA designDNA analysis softwareHigh throughput cloningPooled cloningMammalian tissue cultureTransfection

Required

Ph.D. in molecular biology, synthetic biology, cell biology, virology, protein engineering, genome engineering, or related scientific field with 0-2 years at the Research Scientist I level, 3-5 years at the Research Scientist II level and 5+ years of relevant industry experience at the Sr. Research Scientist level. An equivalent combination of education and experience may be considered. The title and responsibility commensurate with relevant experience.

Experience with transposases, recombinases, CRISPR, TALENs, ZFNs, Prime Editing, Base Editing, or other genome editing tools

Proficiency in bioinformatics/machine learning/computer languages (such as Python) highly valued

Previous experience with functional assays and analyses used to measure success of gene editing/knock-in/knock-outs and genotoxicity, including insertion site characterization, off-target evaluation, and genomic instability assessment

Understanding and hands-on experience with various directed evolution or rational/structure-based protein engineering strategies is a plus

Experience with cloning techniques such as restriction digests, Gibson assembly, transformations, Sanger sequencing, plasmid preps, and DNA design/analysis software. High throughput or pooled cloning work is a plus

Proficiency with mammalian tissue culture and transfection of immortalized cell lines. Experience with various primary cell types is a plus

Demonstrated ability to be independent, self-motivated, as well as work in a highly collaborative setting

Excellent oral and written communication skills, attention to detail, and documentation skills

Excellent critical thinking and analytic skills and great trouble-shooting ability are required

Must be passionate about performing research that may lead to the treatment or cure of human disease

Able to lift to 40 pounds and stand for hours at a time

The position requires a combination of standing or sitting in a laboratory environment as well as walking around the facility

Preferred

Proficiency with bioinformatics/machine learning/computer languages (such as Python)

Experience with various directed evolution or rational/structure-based protein engineering strategies

High throughput or pooled cloning work

Experience with various primary cell types

Multicolor flow cytometry, cell sorting, and FACS analysis software

PCR, qRT-PCR, ddPCR, in vitro transcription and RNA preparation

Library preparation and Next Generation Sequencing on Illumina and nanopore platforms

Lentiviral production and transduction

Protein purification, Western Blot, chromatography

Company

Poseida Therapeutics, Inc.

Poseida Therapeutics is a clinical-stage biopharmaceutical company advancing differentiated cell and gene therapies with the capacity to cure certain cancers and rare diseases.

Founded in 2014

San Diego, California, USA

201-500 employees

http://poseida.com

H1B Sponsorship

Poseida Therapeutics, Inc. has a track record of offering H1B sponsorships. Please note that this does not guarantee sponsorship for this specific role. Below presents additional info for your reference. (Data Powered by US Department of Labor)

Distribution of Different Job Fields Receiving Sponsorship

Trends of Total Sponsorships

2023 (3)

2022 (3)

2021 (2)

2020 (2)